|
KMID : 0903519700130010065
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1970 Volume.13 No. 1 p.65 ~ p.72
|
|
|
N - Terminal Sequences of λ - type Bence Jones Proteins
|
|
|
|
|
Abstract
|
|
|
Two peptides (Im pr-M, Im ch-M) derived from Im λ-type of Bence Jones Protein and one peptide (Ikch-M) from Ik were separated and purified using the Dowex 50×2 column (1×20 ㎝) and Dowex 1×2(0.9×50 ㎝). The buffer solution was composed of 1% pyridine and IM formic acid in Dowex 1×2 column. The blocked N-terminal was examined with ninhydrin reaction before and after alkaline hydrolysis, which was fractionated by Dowex 1×2 column.
Pyrro-glutamic acid in N-terminal residue was identified by comparing with the authentic pyrro-glutamic acid through a high voltage electrophoresis (pH 3.5, 3000 V.) after the peptide Im pr-M (PCA. Ser) was cleavaged at the position of serine with cone. (12 N) HCl and the pyrro-glutamic acid was converted to glutamic acid by treating it with N-NaOH for 116 hours at 27℃.
The substractive method was applied to find out the sequence of peptides and carboxypeptidase A was employed to release C-terminal residue from the peptide.
In present study PCA. Ser in Im Pr-M was isolated from the pronase digested λ-type Bence Jones protein.
The yield of the Im Pr-M was 79.6 percent of its theoretical value, based on the molecular weight of Bence Jones Protein.
Im ch-M (PCA. Ser Val. Leu) was isolated from the chymotrypsin digested λ-type Bence Jones Protein. The yield of the Im ch-M was 72.2 percent. based on the molecular weight of Bence Jones Protein.
Ik ch-M (PCA. Ser. Ala. Leu) was isolated from the chymotrypsin digested λ-type Bence Jones Protein and its yield was 42% based on the molecular weight of Bence Jones Protein.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|